Congresso Brasileiro de Microbiologia 2023

Congresso Brasileiro de Microbiologia 2023

Resumo: 474-1

474-1

ANTIMICROBIAL ACTIVITY OF A COMPOUND ISOLATED FROM THE ETHANOLIC CRUDE EXTRACT OF THE BARK OF Hymenaea martiana Hayne (FABACEAE) AGAINST TOLERANT AND PERSISTENT Staphylococcus spp. ISOLATES FROM GOAT MASTITIS

Autores:

Gutiele do Nascimento do É (UNIVASF - Universidade Federal do Vale do São Francisco) ; Isabelle Caroline Pires (UNIVASF - Universidade Federal do Vale do São Francisco) ; Danillo Sales Rosa (UNIVASF - Universidade Federal do Vale do São Francisco) ; Alisson Teixeira da Silva (UNIVASF - Universidade Federal do Vale do São Francisco) ; Vitor Hugo Penariol Morante Penariol Morante (UNIVASF - Universidade Federal do Vale do São Francisco) ; Maria Aparecida Scatamburlo Moreira (UFV - Universidade Federal de Viçosa) ; Mateus Matiuzzi da Costa (UNIVASF - Universidade Federal do Vale do São Francisco)

Resumo:

Staphylococcus spp. is the most prevalent etiologic agent responsible for causing mastitis in goats. These microorganisms have developed evolutionary adaptations that are able to survive antibiotic treatment. The phenomenon of tolerance and persistence is the ability of bacterial populations and/or subpopulations to survive the action of antimicrobial agents without change in MIC because of decreased physiological and metabolic state. The aim of this study was to evaluate the antibacterial effect of the compound isolated from the crude ethanolic extract of the bark of Hymenaea martiana Hayne against tolerant and persistent Staphylococcus spp. isolates from caprine mastitis. The isolates belonged to the herd of the Teaching, Research and Extension Unit in Caprinoculture of the Federal University of Viçosa, located in the municipality of Viçosa-Brazil (20.7467991 S 43.0193046 W). They were kindly provided to the Laboratory of Animal Microbiology and Immunology – UNIVASF, at a lat. of 09° 23' 55'' and long. of 40° 30' 03'' in Petrolina, Brazil and registered in SisGen with registration number AEB34A6. To perform the experiment, 12 isolates of Staphylococcus spp. were used, being four tolerant S. aureus isolates (Cap 6.1, Cap 6.2, Cap 7.1 and Cap 7.2) and eight persistent S. warneri isolates (Cap 9.2, Cap 10.1, Cap 10.2, Cap 100.1, Cap 100.2, Cap 101.1 and Cap 101.2). In addition, two standard S. aureus strains were adopted: ATCC 25923 and ATCC 33591. Isolates were standardized to establish a correlation of optical density and colony forming unit (OD X CFU/ml) to establish a bacterial population at a concentration of 5.5X106 CFU/ml for subsequent steps. The tests were performed in biological duplicate To evaluate the antibacterial activity of the compound isolated from To evaluate the antibacterial activity of the compound isolated from the extract, broth microdilution methodology was used to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) at the following concentrations: 12.500 μg/mL; 6.250 μg/mL; 3.125 μg/mL; 1.562.5 μg/mL; 781.3 μg/mL; 390.6 μg/mL; 195.3 μg/mL; 97.6 μg/mL. The minimum inhibitory concentration was assessed using 2,3,5-triphenyltetrazolium chloride (TTC). The compound isolated from jatoba bark showed antibacterial activity against 13 (100%) isolates of Staphylococcus spp. with MIC ranging from 390.6 to 781.2 µg/mL and MBC concentration ranging from 390.6 to 1562.5 ug/ml. The isolated compound showed antimicrobial activity for 7 (54%), 5 (38%) and 1 (8%) of the isolates at concentrations of 781.8 μg/mL, 390.6 μg/mL and 1562.5 μg/mL respectively. The compound isolated from the crude ethanolic extract of H. martiana Hayne showed good antimicrobial activity against tolerant and persistent Staphylococcus spp. isolates from caprine mastitis.

Palavras-chave:

goat farming, phytotherapy, slow Growth, dormancy

Agência de fomento:

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001. The Pernambuco Science and Technology Support Foundation (FACEPE).